林学中心团队 (Research)
植物营养学(Plant Nutrition)

主任(PI): 刘坤祥(Kunxiang Liu, PhD)

 

团队成员 (Members): 罗虹(Hong Luo),林泽忠(Zezhong Lin),郑晓微(Xiaowei Zheng),林子炜(Ziwei Lin),罗小琴(Xiaoqin Luo)

PI简介(PI Introduction)

刘坤祥,教授, 博士生导生。1996年于台湾大学植物学系取得理学学士学位,1998年于台湾大学植物学研究所取得理学硕士学位, 2003年于台湾国防医学院生命科学研究所取得理学博士学位,主要以研究拟南芥的硝酸盐吸收分子机制之探讨。 2003年在台湾中央研究院分子生物学研究所进行博士后研究, 2003年到2013年在美国哈佛医学院遗传学研究所及麻塞诸塞州总医院分子生物学研究所进行博士后研究主要研究方向为拟南芥硝酸盐信息传导,2013-2015年在美国麻塞诸塞州总医院分子生物学研究所担任助理研究员。目前主持一项国家自然科学基金面上项目:解析钙离子依赖的蛋白激酶在硝态氮信号传导中的分子调控机理。
邮箱 (Email):khliu@molbio.mgh.harvard.edu

研究领域 (Research focus):

氮素是植物所需要的巨量元素之一,其中氮素获取与否及其利用率的高低是农作物产量高低的关键。大部分植物主要以吸收硝态氮和铵作为氮的来源,然而许多植物都有铵盐毒害问题,因此对大部分的高等植物而言,硝态氮为其最适合的氮肥形式。硝态氮除了作为一个营养素外, 同时也是植物中一种重要的信号分子。硝态氮可快速诱导许多包括参与硝态氮吸收、代谢途径及糖代谢相关的基因表达,并且硝态氮会调控种子休眠,诱导植物根系的发育、叶片生长和开花时间。本课题组主要的研究方向是以拟南芥为模式植物研究硝态氮/氮素信号传导以及铵盐毒害的相关议题。利用传统遗传学,功能基因学以及表观基因组学方法来解析可能参与在硝酸盐信号传导的蛋白/基因。目前将特别针对:1. 解析钙离子依赖的蛋白激酶在硝态氮信号传导中的分子调控机理。2. 解析转录因子在硝态氮信号传导中的分子调控机理。3. 铵盐毒害的分子机制等这些课题进行深入的研究。此外将开展福建省主要林木树种(如毛竹、杉木等)和水稻的硝态氮/氮素信号传导以及铵盐毒害的原生质体细胞研究系统。将利用在拟南芥的研究结果推广到这些经济作物中以期能揭示植物氮素信号传导的调控机制,解决林木铵盐毒害及为林业生产问题提供理论基础。

Nitrogen is one of the macronutrient for plant to growth. Nitrogen availability is the limiting factor for improving crop yield. In plants, nitrate and ammonium are two major nitrogen sources. Because of the ammonium toxicity issue, for most of higher plants, nitrate is the suitable form of nitrogen fertilizer. Nitrate is not only a macronutrient, but also a signaling molecule. Many genes involved in nitrate uptake, assimilation, and sugar metabolism pathway can be induced by nitrate.  Nitrate regulates seed dormancy, root morphology, shoot growth and flowering time. Our research interests are focusing on nitrate/nitrogen signaling and ammonium toxicity issue in Arabidopsis. We will combine traditional genetics, functional genomic, and epigenetics approaches to dissect components involved in nitrate signaling. Particularly, we will focus on 1: Dissecting the molecular function of Calcium-dependent protein kinases(CPK) in nitrate signaling. 2. Elucidating the molecular function of transcription factors in nitrate signaling. 3. Studying of ammonium toxicity in plants. Furthermore, we will establish protoplasts assay system for studying  nitrate/ nitrogen signaling and ammonium toxicity in major forestry species, Moso bamboo, and China fir and important crop, rice, in Fujian. We will apply and extend our study from Arabidopsis into these crop and important forestry species to provide the theoretical basis for crop and forestry production.

                                     

发表文章 (Publications):


      2017

Kun-hsiang Liu , Yajie Niu , Mineko Konishi , Yue Wu , Hao Du , Hoo Sun Chung , Lei Li , Marie Boudsocq , Matthew McCormack , Shugo Maekawa, Tetsuya Ishida , Chao Zhang, Kevan Shokat, Shuichi Yanagisawa and Jen Sheen.(2017) Discovery of nitrate–CPK–NLP signaling in central nutrient–growth networks Nature 545:311-316.

2014

Wu, Hungi-Yi, Liu, Kun-Hsiang, Wang, Yi-Chieh, Wu, Jing-Fen, Chiu, Wan-Ling, Chen, Chao-Ying, Wu, Shu-Hsiang, Sheen, Jen, and Lai, Erh-Min.(2014) AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analysis in Arabidopsis seedlings Plant Methods 10:19.

Before 2014

Liu, Kun-Hsiang, McCormack, Matthew and Sheen, Jen. (2012) Targeted parallel sequencing of large genetically-defined genomic regions for identifying mutations in Arabidopsis Plant Methods 8:12.
        Liu, Kun-Hsiang, and Tsay, Yi-Fang.(2003) Switching between the two action modes of the dual-affinity nitrate transporter CHL1 by phosphorylation EMBO J 22:1005-1013.
        Huang, Nien-Chen, Liu, Kun-Hsiang, Lo, Hau-Jan and Tsay, Yi-Fang.(1999) Cloning and function characterization of an Arabidopsis nitrate transporter gene that encodes a constitutive component of low-affinity uptake Plant Cell 11:1381-1392.
        Liu, Kun-Hsiang, Huang, Chi-Ying and Tsay, Yi-Fang.(1999) CHL1 is a dual-affinity nitrate transporter of Arabidopsis involving in multiple phases of nitrate uptake Plant Cell 11:865-874.