林学中心团队 (Research)
林木表观遗传学(Plant Epigenetics)

主任(PI): 以色列•奥辛(Israel Ausin, PhD)

 

团队成员 (Members): 刘启昆(Qikun Liu,PhD),王亚飞(Yafei Wang,PhD ),徐磷浩(Linhao Xu),张娅欣(Yaxin Zhang)


PI简介(PI Introduction)

Israel Ausin, 教授,博士生导师。1998 年于西班牙阿尔卡拉大学获得理学学士学位;2004年于西班牙马德里自治大学获得植物学博士学位;2005-2011 年在美国加利福尼亚大学(UCLA)Steve E. Jacobsen实验室从事博士后研究。2011-2014在西班牙马德里国家生物科技中⼼心 (CNB-CSIC)担任研究员。

邮箱:israel.ausin@gmail.com

研究领域 (Research focus):

通过遗传,生化,蛋白质组和基因组的手段探索和阐释DNA和组蛋白的表观遗传学修饰原理,及其在基因表达,基因组稳定性 中的作用机制。具体关注DNA和组蛋白的表观遗传修饰对染色质的高级结构的影响,和在细胞分子水平对生物发育的作用。我们以拟南芥为主要的模式研究对象, 同时也关注表观遗传学在作物和裸子林业植物中的应用。

We take the model plant Arabidopsis and gymnosperm tree crops (such as China fir and Norway spruce) as research objects to study epigenetics. Using the genetic, biochemical, proteomic and genomic methods, we want to elucidate the epigenetic modification principles of DNA and histones, and their mechanisms in gene expression and genome stability. We analyze the effect of DNA and histone epigenetic modifications on chromatin structure, and their roles in plant growth and development.

发表文章 (Publications):

2016

Ausin I,Feng S,Yu C, Liu W, Kuo HY, Jacobsen EL, Zhai J, Gallego-Bartolome J, Wang L, Egertsdotter U, Street NR, Jacobsen SE and Wang H. (2016) DNA methylome of the 20-gigabase Norway spruce genome Proc. Nat. Acad. Sci. U. S. A doi/10.1073/pnas.1618019113.

2015

Zhai J, Bischof S, Wang H, Feng S, Lee TF, Teng C, Chen X, Park SY, Liu L, Gallego-Bartolome J, Liu W, Henderson IR, Meyers BC, Ausin I, Jacobsen SE.(2015)A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis Cell 163(2):445-455.
       Wang H, Beyene G, Zhai J, Feng S, Fahlgren N, Taylor NJ, Bart R, Carrington JC, Jacobsen SE, Ausin I.(2015) CG gene body DNA methylation changes and evolution of duplicated genes in cassava Proc Natl Acad Sci U S A 112(44):13729-13734.

Wangsheng Zhu, Israel Ausin, Andrei Seleznev, Belen Mendez-Vigo, F.Xavier Pico, Sridevi Sureshkumar, Vignesh Sundaramoorthi, Dieter Bulach, David Powell, Torsten Seemann, Carlos Alonso-Blanco,Sureshkumar Balasubramanian.(2015) Natural variation identifies ICARUS1, a universal gene required for cell proliferationand growth at high temperatures in Arabidopsis thaliana. PLOS Genetics  e1005085.

Xuehua Zhong, Christopher J. Hale, M. Nguyen, Israel Ausin, Martin Groth, Johnathan Hetzel J, Ajay A Vashisht A, Ian R Henderson, James A Wohlschlegel and Steven E Jacobsen. (2015) DOMAINS REARRANGED METHYLTRANSFERASE3 controls DNA methylation and regulates RNA polymerase V transcript abundance in Arabidopsis PNAS 112: 911-916.
      Yafei Wang, Haiyang Zeng, Xu Zhou, Fei Huang, Wei Peng, Lin Liu, Wentao Xiong, Xue Shi, Meizhong Luo.(2015) Transformation of rice with large maize genomic DNA fragments containing high content repetitive sequences Plant Cell Reports DOI 10.1007/s00299-015-1764-2.
      Yafei Wang, Meizhong Luo.(2015) Random DNA binding selection assay (RDSA) Bio-protocol 5(8):1-5.

2014
      Martin Groth, Hume Stroud, Suhua Feng, Maxim VC Greenberg, Ajay A Vashisht, James A Wohlschlegel, Steven E Jacobsen and Israel Ausin.(2014)  SNF2 chromatin remodeler-family proteins FRG1 and -2 are required for RNA-directed DNA methylation PNAS  11 (49): 17666-17671.
      Yingxiang Wang, Rong Xiao, Haifeng Wang, Zhihao Cheng, Wuxing Li,Genfeng Zhu,Ying Wang, Hong Ma.(2014) TheArabidopsis RAD51 paralogs RAD51B, RAD51D andXRCC2 play partially redundant roles in somatic DNA repair and gene regulation New Phytologist doi:10.1111/nph.12498.
      Haifeng Wang, Chenjiang You, Fang Chang, Yingxiang Wang, Lei Wang, JiQi, Hong Ma.(2014) The analysis of alternative splicing of genes during early flower development in Arabidopsis thaliana revealsstage-regulated expression of isoforms Frontiers in Genetics doi: 10.3389/fgene,00025.

Liu, Q., Wang,F., Axtell, M.J. (2014) Analysis of Complementarity Requirements for PlantmicroRNA Targeting Using a Nicotiana benthamiana Quantitative Transient Assay Plant Cell 26(2): 741-753.
      Brousse, C., Liu,Q., Beauclair, L., Deremetz, A., Axtell, M.J., Bouché, N. (2014) ANon-canonical Plant microRNA Target Site Nucleic Acids Research doi:10.1093/nar/gku157.
      Yafei Wang, Wei Peng, Xu Zhou, Fei Huang, Linyun Shao, Meizhong Luo.(2014) The putative Agrobacterium transcriptional activator-like virulence protein VirD5 may target T-complex to prevent the degradation of coat proteins in the plant cell nucleus The New phytologist 203:1266-1281.

Before 2014
      Jiemeng Liu, Haifeng Wang, Hongxing Yang, YizheZhang, Jinfeng Wang, Fangqing Zhao, Ji Qi .(2013) Composition based classification of short metagenomic sequenceselucidates the landscapes of taxonomic and functional enrichment of microorganisms Nucleic AcidsRes (41)1doi:10.1093/nar/gks828.
      Israel Ausin, Maxim V C Greenberg, Dhirendra K Simanshu, Christopher J Hale, Ajay A Vashisht, Stacey A Simon, Tzuu-fen Lee, Suhua Feng, Sophia D Espanola, Blake C Meyers, James A Wohlschlegel, Dinshaw J Patel, Steven E Jacobsen.(2012) INVOLVED IN DE NOVO 2-containing complex involved in RNA-directed DNA methylation in Arabidopsis PNAS 109(22):8374-8381.
      Israel Ausin, Maxim V C Greenberg, Carey Fei Li, Steven E Jacobsen.(2012) The splicing factor SR45 affects the RNA-directed DNA methylation pathway in Arabidopsis Epigenetics  7(1):29-33.
      Maxim V C Greenberg, Israel Ausin, Simon W L Chan, Shawn J Cokus, Josh T Cuperus, Suhua Feng, Julie A Law, Carolyn Chu, Matteo Pellegrini, James C Carrington, Steven E Jacobsen.(2011)  Identification of genes required for de novo DNA methylation in Arabidopsis Epigenetics  6(3):344-54.
      Angelique Deleris, Maxim V C Greenberg, Israel Ausin, Rona W Y Law, Guillaume Moissiard, Daniel Schubert, Steven E Jacobsen.(2010)  Involvement of a Jumonji-C domain-containing histone demethylase in DRM2-mediated maintenance of DNA methylation EMBO Reports 11(12):950-5.
      Julie A Law, Israel Ausin, Lianna M Johnson, Ajay A Vashisht, Jian-Kang Zhu, James A Wohlschlegel, Steven E Jacobsen.(2010)  A protein complex required for polymerase V transcripts and RNA- directed DNA methylation in Arabidopsis Current Biology 20(10):951-956.
      Israel Ausin, Todd C Mockler, Joanne Chory, Steven E Jacobsen.(2009)  IDN1 and IDN2 are required for de novo DNA methylation in Arabidopsis thaliana Nature Structural & Molecular Biology 16(12):1325-1327.
      Ming, F., Liu,Q., Shi, J., Wang, W., Lu, B. (2009) Strategic Conservation of OrchardGermplasm Based on Indigenous Knowledge and Genetic Diversity: a Case Study ofSour Orange Populations in China J Integr Plant Biol 1:100-106.
      Zhu, Y., Liu, Q., Yan, P., Shao, M., Lu, D., Qian, J. (2007) MethodResearch of DNA Extraction from Mucous Layer Cells of Human Oral Cavity Journal of Healthy Birth & Child Care(China) 2:87-90.
      Israel Ausin, Carlos Alonso-Blanco, Jose M Martinez-Zapater.(2005)  Environmental regulation of flowering International Journal of Developmental Biology 49(5-6):689-705.
      Israel Ausin, Carlos Alonso-Blanco, Jose A Jarillo, Leonor Ruiz-Garcia, Jose M Martinez-Zapater.(2004) Regulation of flowering time by FVE, a retinoblastoma-associated protein Nature Genetics 36(2):162-166.